Investigation of GhFAT Genes Related to Seed Oil Content and Fatty Acid Composition in Gossypium hirsutum L.

Fatty Acyl-ACP thioesterase (FAT) is a key enzyme controlling oil biosynthesis in plant seeds. FATs can be divided into two subfamilies, FATA and FATB according to their amino acid sequences substrate specificity. The Upland cotton genome contains 20 GhFAT genes, amongst which 6 genes were of the GhFATA subfamily 14 GhFATB subfamily. are unevenly distributed on chromosomes. have 5 or 7 exons exons. All proteins conserved Acyl-ACP_TE domain PLN02370 super family, typical characteristics thioesterases. Analyses expression level GhFATs compositions fatty 5–60 days-post-anthesis seeds showed that ratio saturated acids unsaturated was consistent with profile GhFATB12, GhFATB3, GhFATB10; monounsaturated polyunsaturated GhFATA3. contents mature cottonseeds positively correlated palmitic linolenic as well seed vigor. These results provide essential information for further exploring role(s) specific determining cottonseed compositions.